ࡱ> `b_5@ gbjbj22 8XXg6666666Jrrrr,<Jv_2^^^^^^^$`Rb_6555_660_ 566^ 5^ , 7 2Y66] $rVrK [.^F_0v_K[ncUc\]JJ6666c6]<0" 8T__JJd JJLab Safety Check List General Lab Safety The location of and the content of my departments Emergency Response Plan. The location of the chemical spill kit and how to use it. The location of the first aid kit and can access it easily. The location of the safety shower and eyewash and how to check them and use them. The proper operation of a chemical fume hood. The proper operation and maintenance of all of the other laboratory equipment (besides the chemical fume hood) in my lab. Personal Protective Equipment Use of safety glasses, goggles, and face shields when working in the laboratory. Use of lab coats, aprons, and other protective clothing when working in the laboratory. Use of latex, nitrile, or other gloves and how to choose the right glove for job. Full coverage shoes with good sole grips are worn in the lab and that no open-toed shoes (sandals) are allowed. Before using respiratory protection for work in the lab, supervisor has been consulted and Environmental Health and Safety has been contacted for information on the Respiratory Protection Program. Before working around high noise producing equipment such as sonicators or grinders, ask supervisor about hearing protection and the Hearing Conservation Program. Removing PPE before leaving the lab. Housekeeping and Hygiene Keeping walkways and access to safety equipment clear of obstructions. Keeping all work surfaces and benches free of clutter to reduce the risk of spills and accidents. Addressing Spills immediately and correctly. Mechanical pipetting devices are used and mouth pipetting is not allowed. Food and beverages are not permitted in the laboratory, nor are they to be stored in laboratory refrigerators or freezers. All laboratory refrigerators, freezers, microwaves are clearly labeled, e.g., non-flammable storage only; no food or beverages. Chemical Safety: Chemicals must be stored properly, taking the following into account: Compatibility Labeling Capping/sealing Container type and condition Liquid chemicals shall be stored below eye level. Proper dating, storage, use, and disposal procedures for the chemicals in my lab must be followed. Glass containers of chemicals are not stored on the floor. Lab Waste Disposal The appropriate hazardous waste disposal procedures for both chemical and biological materials must be followed. Broken laboratory glass must be separated from the regular or municipal waste stream and placed in properly labeled and lined boxes that can be easily closed and sealed before disposal. Broken glassware that is contaminated with biological materials must be placed in a lined box marked with the biohazard symbol. All needles and sharps must be disposed of in a puncture resistant and properly labeled sharps container. Compressed Gases Compressed gas cylinders are adequately secured even when empty and are stored by compatibility. Hand trucks are used to safely transport gas cylinders. Gas cylinders are stored away from heat sources. The connection between the regulator and the gas cylinder is leak tested after installation and before each use. Gas cylinders without regulators must be capped (even when empty). The contents of the gas cylinders must be clearly labeled. The hydrostatic tests on the gas cylinders must be current (cylinders have not been stored more than 5-10 years). Electrical Safety Access to the circuit breaker panel is unobstructed. Ground Fault Circuit Interrupters (GFCI) are used when within six feet of a water source. Electrical cords must be checked routinely for signs of fraying or damage. Lab instruments and appliances are plugged directly into electrical outlets (UL rated fuse protectors may be used when there is a lack of electrical outlets, but extension cords cannot be used as a permanent power supply). Fire Safety A fire extinguisher is located near or in the laboratory and training on how to use it has been provided. Containers of flammable liquids are grounded during transfer. A maximum of 10 gallons flammable liquids are stored in the lab, the excess is stored in a safety cabinet, up the maximum allowed per control area. Contact Environmental Health and Safety for more information. Flammable liquids over one gallon are stored in safety cans. Sprinkler heads are not blocked. Biological Materials Recombinant DNA experiments, BSL2-BSL3 experiments, and any other experimental activity utilizing biological materials that may pose a risk to humans or the environment must be reviewed and approved by the Biosafety Committee via a Memorandum of Understanding (MUA) before work can begin. Work with materials listed as Select Agents or Toxins by Health and Human Services department of the Centers for Disease Control (CDC) or the Animal and Plant Health Inspection Service (APHIS) must be registered through either agency as appropriate and reviewed and approved by the University Biosafety Committee before materials can be acquired and before work can begin. Laboratory personnel should receive appropriate training in biosafety for laboratory personnel and/or select agents and toxin regulatory requirements as appropriate to their activities in the lab. Radiation Safety Laboratory personnel should receive radiation safety training appropriate to their level of involvement with working with or around radiation. 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